All specimens submitted for testing must be properly labeled with patient name, MRN/SSN, date collected, and name of physician. Specimens must be accompanied by a CIS requisition or a DMCUL Molecular Genetics requisition form.
Causes for Sample Rejection:
We cannot accept serum, frozen whole blood, or samples that have been mis-labeled or are without labels.
Peripheral blood: 3-6 ml whole blood into lavender (EDTA) top tubes (preferably in 2X3.5 ml tubes) of whole blood. Sample amount for infants: 1-2 ml whole blood collected in lavender (EDTA) tubes. Invert several times to mix with anticoagulant. DO NOT FREEZE!
Discard the first 1-2 ml of aspirated amniotic fluid to reduce the risk of maternal cell contamination. Obtain 10-20 ml amniotic fluid by amniocentesis. Two or three sequentially numbered aliquots are requested. Send to Cytogenetics Laboratory
immediately after collection, preferably by overnight delivery. If sending cultured amniotic fluid cells, 1-2 T-25 flasks are required. Keep specimen at room temperature. DO NOT FREEZE
Chorionic Villus: Obtain 20-30 mg of chorionic villis (10 mg minimum) and place into sterile tube containing transport medium. Send to Cytogenetics Laboratory immediately after collection, preferably by overnight delivery. If sending cultured chorionic villus cells, 1-2 T-25 flasks are required. Keep specimen at room temperature. DO NOT FREEZE!
Paraffin embedded tissue: Paraffin blocks with the highest percent of tumor to normal ratio are forward directly to the laboratory (preferably >30% of embedded tissue is tumor tissue); up to 40 microns will be used to isolate genomic DNA from the embedded tissue. The paraffin block will be returned to sender after the test is complete.
Bone Marrow: 1-2 ml freshly aspirated bone marrow into lavender (EDTA) top tube. Invert several times to mix with anticoagulant. DO NOT FREEZE!
Submit 40-60 mg fresh or flash frozen tissue. For skin specimens, punch biopsy is adequate for most tests. Please contact the laboratory for specific test requirements.