MLH1 Promoter Methylation studies

Order Code: 335562

CPT Codes: 81479, G0452

Indication: Help determine whether a tumor more likely occurred as a result of sporadic or inherited pathogenic variants.

Required Patient Information: Clinical Diagnosis, Surgical Pathology report

Turn Around Time: 10 days

Specimen type: Paraffin embedded tissue 

Methodology: The C region of the MLH1 promoter is amplified by multiplex PCR with two fluorescently-labeled primer pairs: one specific for the methylated allele, and one specific for the unmethylated allele. Individuals with a normal methylation pattern will have amplification of the unmethylated allele only.